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Tumor Suppressor miR-613 Alleviates Non-Small Cell Lung Cancer Cell via Repressing M2 Macrophage Polarization 
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Analysis of miR-613 on NSCLC <t>cell</t> <t>proliferation</t> and apoptosis. miR-613 mimic was transfected into H1650 cells. (a) Evaluation of H1650 cell viability by cell counting kit-8 (CCK-8). (b) <t>Edu</t> method was conducted to assess H1650 cell proliferation (scale bar: 100 μ m). (c) H1650 cell apoptosis was determined by flow cytometry. (d) The levels of apoptosis-related proteins Bax, Bcl2, and cleaved caspase-3 were tested by Western blot. ∗∗ P < 0.01, ∗∗∗ P < 0.001 vs. NC-mimic. NC: negative control.
Tumor Suppressor miR-613 Alleviates Non-Small Cell Lung Cancer Cell via Repressing M2 Macrophage Polarization 
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miR-613 regulates NSCLC cell growth by repressing M2 macrophage polarization. Macrophages with different treatments (as in ) were co-cultured with H1650 cells. H1650 cells were grouped: H1650+M NCmimic , H1650+M IL-4 + NCmimic and H1650+M IL-4+miR-613mimic . (a) The schematic diagram of the co-culture system. (b) CCK-8 was conducted to assess H1650 cell viability. (c) Evaluation of H1650 <t>cell</t> <t>proliferation</t> by the <t>Edu</t> method (scale bar: 100 μ m). (d) Detection of H1650 cell apoptosis by flow cytometry. (e and f) H1650 cell migration was tested using Transwell (scale bar: 200 μ m) and wound-healing analyses (scale bar: 400 μ m). (g) H1650 cell invasion was measured by Transwell (scale bar: 200 μ m). ∗∗∗ P < 0.001 vs. H1650+M NCmimic . ## P < 0.01, ### P < 0.001 vs. H1650+M IL-4 + NCmimic .
Tumor Suppressor miR-613 Alleviates Non-Small Cell Lung Cancer Cell via Repressing M2 Macrophage Polarization Journal of Oncology, 2023 Mar 06
"Analysis of miR-613 on NSCLC <t>cell</t> <t>proliferation</t> and apoptosis. miR-613 mimic was transfected into H1650 cells. (a) Evaluation of H1650 cell viability by cell counting kit-8 (CCK-8). (b) <t>Edu</t> method was conducted to assess H1650 cell proliferation (scale bar: 100 μ m). (c) H1650 cell apoptosis was determined by flow cytometry. (d) The levels of apoptosis-related proteins Bax, Bcl2, and cleaved caspase-3 were tested by Western blot. ∗∗ P < 0.01, ∗∗∗ P < 0.001 vs. NC-mimic. NC: negative control. "
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